Enssure Dengue Combo Assay Kit

Dengue, a flavivirus, is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. Symptoms typically manifest three to fourteen days post-infection. The dengue NS1 test, which stands for Dengue non-structural protein 1, is utilized for early dengue fever diagnosis by detecting the presence of dengue NS1 antigen in the blood. This test allows rapid detection on the first day of fever, preceding the appearance of antibodies by 5 or more days. In primary Dengue infections, IgM antibodies become detectable 3 to 5 days after the onset of fever and usually persist for 30 to 90 days. In endemic regions, most Dengue patients experience secondary infections, leading to elevated levels of specific IgG antibodies, either before or concurrently with the IgM response.

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Dengue Combo (Dengue NS1 + Dengue IgG/IgM)

Product Information

Intended Use

Dengue Combo (Dengue NS1+Dengue I gG/I gM) t e s t i s an immunochromatographic assay for the qualitative detection Dengue NS1 antigen and IgG/IgM Antibodies to Dengue v irus in human serum/plasma/whole blood.

Principle 

Dengue NS1 : The Rapid Dengue NS1 Antigen test involves adding a blood sample to the device, where it reacts with gold-conjugated Dengue NS1 antibodies. This forms a complex that moves onto a nitrocellulose membrane, creating a colored Test Line if Dengue NS1 antigens are present. The Control Line, generated by the reaction with goat anti-rabbit IgG, serves as a procedural control, indicating a valid test when both lines appear.

Dengue IgG/IgM : The Dengue IgG/IgM Antibody test employs a lateral flow chromatographic immunoassay. When the sample containing Dengue-specific IgM and/or IgG antibodies is added, it reacts with colloidal gold particles carrying Dengue-specific recombinant antigens, forming a complex. This complex then produces colored bands on the test and control lines, with the appearance of both indicating a positive result, while the control line alone signifies a negative result, ensuring the test's procedural and reagent integrity.

 Testing Procedure Dengue NS1